HTR3A Primary Antibody
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purified recombinant fragment of human HTR3A (AA: extra 24-157) expressed in E. Coli.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)
Figure 3:Western blot analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HTR3A mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HTR3A mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.