Mouse Monoclonal Antibody to HSP70

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Clone#2E4F10B11
Entrez GenelD3308
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian.
MW94.3kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
642-841
Catalog#: 32244
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Western Blot
Figure 9:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)
Figure 9:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.
Product Overview
Description

HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Vulvovaginitis and Babesiosis. Among its related pathways are Cellular response to heat stress and Mechanisms of CFTR activation by S-nitrosoglutathione (normal and CF). An important paralog of this gene is HSPA4L.

References (references)
References (references)1.J Biol Chem.2020 Jun 12;295(24):8302-8324.2.Biol Chem.2020 Oct 25;401(11):1233-1248.