Mouse Monoclonal Antibody to HSP70
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52 |
| Clone# | 2E4F10B11 |
| Entrez GenelD | 3308 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian. |
| MW | 94.3kDa |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 642-841 | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Western Blot
Figure 9:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.