Mouse Monoclonal Antibody to HSP70
Item Information
Catalog #
Size
Price
Description
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Vulvovaginitis and Babesiosis. Among its related pathways are Cellular response to heat stress and Mechanisms of CFTR activation by S-nitrosoglutathione (normal and CF). An important paralog of this gene is HSPA4L.
Product Overview
Entrez GenelD
3308
Aliases
RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Clone#
2E4F10B11
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem.2020 Jun 12;295(24):8302-8324.2.Biol Chem.2020 Oct 25;401(11):1233-1248.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e9059080a0cd9f06dbddff4f369b05b1e03ae2a3e9710fe07a64b7bf41733370.jpg?v=1666706059)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3560df8e2369d9cb0bf3132c435b0330776bfbf7e2be62ab0ff27fb8ee64155b.jpg?v=1666706059)
Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c761761dc54952d6a5a45af3699b4ab6529e4c83aa0540f1cca40c44ab984596.jpg?v=1666706059)
Figure 4:Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fe5faaf0dd0062447f852334179674125739a7857165f8ab7c87d72585b12168.jpg?v=1666706059)
Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/213e2dd461854e281a1f1029eefacd138c0df377da23b87860ed84367621561a.jpg?v=1666706059)
Figure 6:Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 7:Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2fc9ef4ada398c5a2f649dcbab51475521df13ed2f36dfe8356f14e97f88e396.jpg?v=1666706059)
Figure 7:Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP70 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fa1843a788b56cc54e27db3c0210161d4f75e19ae67ca2bc4e58994b9c4f564d.jpg?v=1666706059)
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Western Blot
![Figure 9:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/366df5b8a6e3f1f2d170e58194ec022077b8a8f7081828e4d4726c893b3a5247.jpg?v=1666706059)
Figure 9:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f7860d9b8dd08a7d789ec3a0b6c14faceddbbeac8472c4db1c94e41523eae1bb.jpg?v=1666706059)
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.