HSF1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.

Product Overview

Entrez GenelD

3297

Aliases

HSTF1

Clone#

4D5F4

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human HSF1 (AA: 256-359) expressed in E. Coli.

Formulation

Purified antibody from tissue culture in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cell. 2012 Aug 3;150(3):549-62.
2. Cancer Lett. 2012 May 28;318(2):145-53.

Product Image

Western Blot

Figure 1: Western blot analysis using HSF1 mAb against human HSF1 (AA: 256-359) recombinant protein. (Expected MW is 36.5 kDa)

Western Blot

Figure 2: Western blot analysis using HSF1 mAb against HEK293 (1) and HSF1 (AA: 256-359)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of A431 cells using HSF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis