HLA-DPA1

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDPA1; PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DPA; HLA-DP1A; HLA-DPB1
Clone#2B1A10
Entrez GenelD3113
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human HLA-DPA1 (AA: 29-209) expressed in E. Coli.
MW29.3kDa
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
extra(29-209)
Catalog#: 32424
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HLA-DPA1 mAb against human HLA-DPA1 (AA: 29-209) recombinant protein. (Expected MW is 23.7 kDa)
Figure 2:Western blot analysis using HLA-DPA1 mAb against human HLA-DPA1 (AA: 29-209) recombinant protein. (Expected MW is 23.7 kDa)
Western Blot
Figure 3:Western blot analysis using HLA-DPA1 mAb against HEK293-6e (1) and HLA-DPA1 (AA:29-209)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using HLA-DPA1 mAb against HEK293-6e (1) and HLA-DPA1 (AA:29-209)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HLA-DPA1 mouse mAb against Raji(1) cell lysate.
Figure 4:Western blot analysis using HLA-DPA1 mouse mAb against Raji(1) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Jurkat cells using HLA-DPA1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using HLA-DPA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded gastric cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded gastric cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Product Overview
Description

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

References (references)
References (references)1.HLA. 2021 Dec;98(6):577-578.
2.HLA. 2022 Jan;99(1):74-75.