HK1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
ProductOrderH
Clone#7A7
Entrez GenelD3098
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HK1 expressed in E. Coli.
MW102kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
316-410
Catalog#: 30084
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) using HK1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) using HK1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

References (references)
References (references)1. Cell. 2005 Sep 23;122(6):957-68.
2. J Biomed Sci. 2007 Mar;14(2):195-202.
3. J Neural Transm. 2009 Mar;116(3):275-89.