HIST2H4A(20Me3) Primary Antibody

Item Information
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Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Product Overview
Entrez GenelD
8370
Aliases
H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Clone#
7A2A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide of human HIST2H4A (AA: GGAKRHRK(Me3)VLRDNIQ) .
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Virol. 2011 Dec;85(24):13234-52.
2.Mol Cell. 2011 Dec 23;44(6):918-27.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HIST2H4A(20Me3) mouse mAb against THP-1 (1), Jurkat (2), K562 (3), NIT/3T3 (4), PC-12 (5), Hela (6), MCF-7 (7), and A431 (8) cell lysate.
Figure 2:Western blot analysis using HIST2H4A(20Me3) mouse mAb against THP-1 (1), Jurkat (2), K562 (3), NIT/3T3 (4), PC-12 (5), Hela (6), MCF-7 (7), and A431 (8) cell lysate.
Flow cytometric
Figure 3:Flow cytometric analysis of Raji cells using HIST2H4A(20Me3) mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of Raji cells using HIST2H4A(20Me3) mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.