Mouse Monoclonal Antibody to HIF2a
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73 |
| Clone# | 1H3E12 |
| Entrez GenelD | 2034 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli. |
| MW | 96.5kDa |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 680-870 | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.