Mouse Monoclonal Antibody to HIF2a
Item Information
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Price
Description
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
Product Overview
Entrez GenelD
2034
Aliases
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Clone#
1H3E12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Nat Commun. 2020 Oct 6;11(1):5023. 2,Blood Cells Mol Dis. 2020 Sep;84:102446.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.