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Mouse Monoclonal Antibody to HIF2a
Item Information | ||
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Catalog # | Size/Concentration | Price |
90011 | 1.0mg/ml | $376.00 |
Specification | |
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Aliases | HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73 |
Clone# | 1H3E12 |
Entrez GeneID | 2034 |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Host | Mouse |
Isotype | IgG1 |
Immunogen | Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli. |
MW | 96.5kDa |
Application | |
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ELISA | 1/10000 |
ICC (Immunocytochemistry) | 1/200 - 1/1000 |
IHC_P (Immunohistochemistry) | 1/200 - 1/1000 |
FCM (Flow Cytometry) | 1/200 - 1/400 |
WB (Western Blot) | 1/500 - 1/2000 |
Sequence |
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680-870 |
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)
Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis

Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).
Flow cytometric analysis

Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.
Description | This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009] |
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References (references) | 1,Nat Commun. 2020 Oct 6;11(1):5023. 2,Blood Cells Mol Dis. 2020 Sep;84:102446. |
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