Mouse Monoclonal Antibody to HIF2a
Item Information
Catalog #
Size
Price
Description
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
Product Overview
Entrez GenelD
2034
Aliases
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Clone#
1H3E12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Nat Commun. 2020 Oct 6;11(1):5023. 2,Blood Cells Mol Dis. 2020 Sep;84:102446.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e7445be28006cf25e9aa9dfbd0b7831065eb84cf17dce3c5304d3d98dcf2ea39.jpg?v=1666706164)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/76e33a530cdbdcc5a8bec0c1632247b761dc938ac498d7c8f9fc6c83fd7b45d8.jpg?v=1666706164)
Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)
Immunofluorescence analysis
![Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0352d7b94edb0c5fbaf3ccf6c86e0fbafdcab4ff16320bf356d227f0f43d3bd1.jpg?v=1666706164)
Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/213da7ee3ec35ce0813a9245e80d756cb41e2edab0b2d2a8929d726b7573d693.jpg?v=1666706164)
Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a961af911050031b6a31993ea19dc4bebb7390742d467879a9ce45d8b9321da2.jpg?v=1666706164)
Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b3d11179edd49e5194f690391aa0e4fc939b36554d9b89b1f7bb58c7e0d25b85.jpg?v=1666706164)
Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6cc5e4ddda4e0796f7ae316d7cf29f3fbcc1b21627c71bb6d57b8a2c8cdebd4d.jpg?v=1666706164)
Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.