Mouse Monoclonal Antibody to HIF2a

Item Information
Catalog # Size/Concentration Price
90011 1.0mg/ml $358.00
Specification
AliasesHLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Clone#1H3E12
Entrez GeneID2034
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.
MW96.5kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
680-870
Catalog
90011

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Images

Elisa

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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

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Figure 2:Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)

Immunofluorescence analysis

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Figure 3:Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis

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Figure 4:Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).

Flow cytometric analysis

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Figure 5:Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).

Immunohistochemical analysis

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Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.

Immunohistochemical analysis

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Figure 7:Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.

Product Overview
Description

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

References (references)
References (references) 1,Nat Commun. 2020 Oct 6;11(1):5023. 2,Blood Cells Mol Dis. 2020 Sep;84:102446.

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