HER-2 Primary Antibody
Item Information
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Size
Price
Description
The C-erbB-2 (HER-2/neu) gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Amplification or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. High levels of c-erbB-2 were associated with estrogen receptor (ER) and progesterone receptor negativity. Overexpression of the c-erbB-2 oncogene has been shown to be associated with poor prognosis in ovarian and breast cance, The level of increased Neu expression can be a predictor of disease prognosis
Product Overview
Entrez GenelD
2064
Aliases
HER-2, C-erB-2, erbB-2
Clone#
9B9D8
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of HER-2 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. RR Mehta, JH McDermott, TJ Hieken, et al. J. Clin. Oncol. 1998;16:2409 - 2416.
2. Hideko Y, Vered S, and Daniel F.H, et al. J. Clin. Oncol.2001;19:2334 - 2356.
3. Magali F, Kamel H, Cécile B, et al. Clinical Cancer Research. 2000;6:4745-4754.
2. Hideko Y, Vered S, and Daniel F.H, et al. J. Clin. Oncol.2001;19:2334 - 2356.
3. Magali F, Kamel H, Cécile B, et al. Clinical Cancer Research. 2000;6:4745-4754.
Product Image
Western Blot
Figure 1: Western blot analysis using HER-2 mouse mAb against truncated HER-2 recombinant protein.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast intraductal carcinama tissue(A) and breast infiltrating ductal carcinama tissue(B) showing membrane localization using HER-2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.