HDAC4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Product Overview
Entrez GenelD
9759
Aliases
HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
Clone#
7E2E6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HDAC4 (AA: 456-592) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Res. 2013 Feb 15;73(4):1386-99.
2.Am J Hum Genet. 2010 Aug 13;87(2):219-28.
2.Am J Hum Genet. 2010 Aug 13;87(2):219-28.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using HDAC4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.