Mouse Monoclonal Antibody to HDAC1
Item Information
Catalog #
Size
Price
Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Product Overview
Entrez GenelD
3065
Aliases
HD1; RPD3; KDAC1; GON-10; RPD3L1
Clone#
1B6A7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Commun Signal. 2019 Jul 29;17(1):86. 2.Hum Pathol. 2019 Mar;85:194-201.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8321225467fbc7399ede05f4b236fbfb00745a0d1b06d95700f5f8144b701cbd.jpg?v=1666705447)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c6486ac6702cfcaaa731021e9ea08dffe133f112dceb9df9e158b0b6410f7536.jpg?v=1666705447)
Figure 2:Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)
Western Blot
![Figure 3:Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 1-482)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bb00115f09d08688c80356274790e9c801221ddeb52048bfde9789dce924c8fc.jpg?v=1666705447)
Figure 3:Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 1-482)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using HDAC1 mouse mAb against NIH/3T3 (1), Hela (2), Raw264.7 (3), K562 (4), Jurkat (5), C6 (6), and Raji (7) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/883e50e85c4a255021c3dd2bbe0b6aeeecb575f5fc552ea247e6170da9fb8a00.jpg?v=1666705447)
Figure 4:Western blot analysis using HDAC1 mouse mAb against NIH/3T3 (1), Hela (2), Raw264.7 (3), K562 (4), Jurkat (5), C6 (6), and Raji (7) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Raji cells using HDAC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cc8cb90873fc979cb0b9481fa0d27329aee42c3b29b971ceaecf4f03fa1faa80.jpg?v=1666705448)
Figure 5:Flow cytometric analysis of Raji cells using HDAC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using HDAC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/dcb93015a2cd3517bfc8f9156a9ad62acbe59c568a978a670d7482d17b01f7c8.jpg?v=1666705448)
Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using HDAC1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HDAC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1245512fb85366fec4f65ac43e0cbaf71b06d7da53cd5191fc1328dff740fb74.jpg?v=1666705448)
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HDAC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.