HAUSP Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesTEF1; HAUSP; USP7
ProductOrderH
Clone#5F11
Entrez GenelD7874
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HAUSP expressed in E. Coli.

MW128kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
WB (Western Blot)1/500 - 1/2000
Sequence
N
Catalog#: 20428
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate.
Figure 1: Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate.
Product Overview
Description

USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

References (references)
References (references)1. Cell Death Differ. 2007 Jul;14(7):1350-60.
2. Cancer Cell. 2007 Oct;12(4):342-54.
3. Blood. 2009 Apr 2;113(14):3264-75.