HAS2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
ProductOrderH
Clone#4E7
Entrez GenelD3037
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HAS2 (AA: 67-170) expressed in E. Coli.
MW63.5kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/100 - 1/500
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
67-170
Catalog#: 30417
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Images
Western Blot
Figure 1: Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)
Figure 1: Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 2: Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.
Figure 2: Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HAS2 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HAS2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.

References (references)
References (references)1.Cancer Res. 2012 Jan 15;72(2):537-47. 2.J Biol Chem. 2011 Sep 23;286(38):33632-40.