H2AFX

Item Information

Catalog #

Size

Price

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Product Overview

Entrez GenelD

3014

Aliases

H2AX; H2A.X; H2A/X

Clone#

4A2D5

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human H2AFX expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

References

1.Radiat Res. 2021 Mar 1;195(3):244-252.
2.Mutat Res Genet Toxicol Environ Mutagen. 2020 Aug-Sep;856-857:503220.

Product Image

Western Blot

Figure 1:Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.

Immunohistochemical analysis

Figure 2:Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis