GUCY1A3 Primary Antibody
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Description
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using GUCY1A3 mAb against human GUCY1A3 recombinant protein. (Expected MW is 47.2 kDa)
Western Blot
Figure 2: Western blot analysis using GUCY1A3 mAb against HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Jurkat (2) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 5: Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.