GSTP1 Primary Antibody
Item Information
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Size
Price
Description
GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.
Product Overview
Entrez GenelD
2950
Aliases
PI; DFN7; GST3; FAEES3
Clone#
3F2C2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GSTP1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Kimihiko Satoh, Ken Itoh, Masayuki Yamamoto. 2002. Carcinogenesis. 23: 457 - 462.
2. Xiaohui Lin,William G. Nelson.2003.Cancer Research. 63: 498-504.
2. Xiaohui Lin,William G. Nelson.2003.Cancer Research. 63: 498-504.
Product Image
Western Blot
Figure 1: Western blot analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).
Western Blot
Figure 2: Western blot analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Confocal Immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 5: Confocal Immunofluorescence analysis of PC-3 cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 6: Flow cytometric analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.