GSK3B Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesGSK-3β; GSK3-beta
ProductOrderG
Clone#3D10
Entrez GenelD2932
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human GSK3B expressed in E. Coli.
MW46kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
292-432(R&D)
Catalog#: 30006
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Images
Western Blot
Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.
Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple).
Product Overview
Description

Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3

References (references)
References (references)1. EMBO J. 1998 Mar 2;17(5):1371-84.

2. Curr Biol. 2001 Jan 9;11(1):44-9.

3. Cancer Lett. 2003 Sep 25;199(2):201-8.