GSK3 alpha Primary Antibody

Item Information

Catalog #

Size

Price

Description

GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.

Product Overview

Entrez GenelD

2931

Aliases

DKFZp686D0638; GSK3A

Clone#

6G2F1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of GSK3 alpha expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Bradley W. Doble and James R. Woodgett. J. Cell Sci., Apr 2003; 116: 1175.
2. C. Laura Sayas , Aafke Ariaens, Bas Ponsioen. Mol. Biol. Cell, Apr 2006; 17: 1834 - 1844.

Product Image

Western Blot

Figure 1: Western blot analysis using GSK3 alpha mouse mAb against truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and skin carcinoma (right), showing cytoplasmic localization using GSK3 aphpa mouse mAb with DAB staining.

For Research Use Only. Not for use in diagnostic procedures.