GRIN3B Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a subunit of an N-methyl-D-aspartate (NMDA) receptor. The encoded protein is found primarily in motor neurons, where it forms a heterotetramer with GRIN1 to create an excitatory glycine receptor. Variations in this gene have been proposed to be linked to schizophrenia.
Product Overview
Entrez GenelD
116444
Aliases
NR3B; GluN3B
Clone#
2A8E11
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GRIN3B (AA: 135-276) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2015 Mar 13;10(3):e0116319.
2.Psychiatry Res. 2014 Aug 30;218(3):356-8.
2.Psychiatry Res. 2014 Aug 30;218(3):356-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GRIN3B mAb against human GRIN3B (AA: 135-276) recombinant protein. (Expected MW is 40.8 kDa)
Western Blot
Figure 3:Western blot analysis using GRIN3B mAb against HEK293 (1) and GRIN3B (AA: 135-276)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of SH-SY5Y cells using GRIN3B mouse mAb (green) and negative control (red).
Flow cytometric
Figure 5:Flow cytometric analysis of SK-N-SH cells using GRIN3B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.