Mouse Monoclonal Antibody to GPR15
Item Information
Catalog #
Size
Price
Description
This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
2838
Aliases
BOB
Clone#
2C2A8
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human GPR15 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biomarkers. 2019 May;24(3):217-224. 2,BMC Pulm Med. 2017 Nov 28;17(1):159.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPR15 mAb against human GPR15 (AA: extra mix) recombinant protein. (Expected MW is 38.3kDa)
Western Blot
Figure 3:Western blot analysis using GPR15 mAb against HEK293-6e (1) and GPR15 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Raji cells using GPR15 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using GPR15 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of THP-1 cells using GPR15 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using GPR15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.