GFP Primary Antibody

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Description

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Product Overview
Clone#
4B10B2
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of GFP expressed in E. Coli.
Formulation
Purified antibody in PBS containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404.
2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30.
3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68.
Product Image
Western Blot
Figure 1: Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
Figure 1: Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
Western Blot
Figure 2: Western blot analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).
Figure 2: Western blot analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).
Immunohistochemical analysis
Figure 3: Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .
Figure 3: Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .
Flow cytometric
Figure 4: Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .
Figure 4: Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 20144

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