GFAP Primary Antibody
Item Information
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Price
Description
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.Tissue specificity: Expressed in cells lacking fibronectin.ABCAM:It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves.In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Product Overview
Entrez GenelD
2670
Aliases
FLJ45472; GFAP
Clone#
6A6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GFAP expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Acta Neuropathol. 2009 Jun;117(6):667-75.
2. Schizophr Res. 2009 Jul;112(1-3):54-64.
2. Schizophr Res. 2009 Jul;112(1-3):54-64.
Product Image
Western Blot
Figure 1: Western blot analysis using GFAP mouse mAb against A431 (1), SK-N-SH (2) and PC12 (3) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues using GFAP mouse mAb with DAB staining
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of paraffin-embedded lobe of brain tissues using GFAP mouse mAb (green).
For Research Use Only. Not for use in diagnostic procedures.