GCK Primary Antibody
|Aliases||GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human GCK expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using GCK mAb against human GCK (AA: 1-198) recombinant protein. (Expected MW is 48.2 kDa)
Figure 2: Western blot analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate.
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
|References (references)||1. Mol Endocrinol. 2009 Dec;23(12):1983-9. |
2. Int J Mol Med. 2009 Aug;24(2):233-46.