GABPA Primary Antibody
Item Information
Catalog #
Size
Price
Description
GABPA: GA binding protein transcription factor, alpha subunit 60kDa. It is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, it may play a role in the Down Syndrome phenotype.
Product Overview
Entrez GenelD
2551
Aliases
NFT2; NRF2
Clone#
8C1B10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Science. 1998 Feb 13;279(5353):1037-41.
2. J Biol Chem. 1999 Dec 10;274(50):35475-82.
3. EMBO J. 2000 Feb 15;19(4):683-90.
2. J Biol Chem. 1999 Dec 10;274(50):35475-82.
3. EMBO J. 2000 Feb 15;19(4):683-90.
Product Image
Western Blot
Figure 1: Western blot analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.
Western Blot
Figure 2: Western blot analysis using GABPA mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of Hela cells using GABPA mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.