G6PD Primary Antibody

Item Information
Catalog # Size/Concentration Price
30284 100μl $341.00
Specification
AliasesG6PD1
Product OrderG
Clone#5E12
Entrez GeneID2539
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human G6PD expressed in E. Coli.\
MW59kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
275-515
Catalog
30284
$341.00
-
+

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Images

Western Blot

Cell Culture Products
Figure 1: Western blot analysis using G6PD mAb against human G6PD (AA: 275-515) recombinant protein.(Expected MW is 53.1 kDa)

Western Blot

Cell Culture Products
Figure 2: Western blot analysis using G6PD mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate.

Immunohistochemical analysis

Cell Culture Products
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using G6PD mouse mAb with DAB staining.

Immunohistochemical analysis

Cell Culture Products
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using G6PD mouse mAb with DAB staining.

Flow cytometric

Cell Culture Products
Figure 5: Flow cytometric analysis of MCF-7 cells using G6PD mouse mAb (green) and negative control (red).

Elisa

Cell Culture Products
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer -
B1/2 Esophageal Cancer +
C1/2 Esophagus -
D1/2 Esophagus +
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach -
H1/2 Stomach +
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver -
D5/6 Renal cell Carcinoma +
E5/6 Kidney +
F5/6 Pancreas -
G5/6 Prostate Cancer +
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
C7/8 Lung +
D7/8 Brain +
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar +
H7/8 Skeletal muscle +
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial +
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Product Overview
Description This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
References (references)
References (references) 1. Science. 2009 Dec 11;326(5959):1546-9.
2. Immunol Invest. 2009;38(6):551-9.

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