FYN Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | SLK; SYN; MGC45350 |
| ProductOrder | F |
| Clone# | 2A10 |
| Entrez GenelD | 2534 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human FYN expressed in E. Coli. |
| MW | 61kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 7-176 | |
Western Blot
Figure 1: Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);