FYN Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Product Overview

Entrez GenelD

2534

Aliases

SLK; SYN; MGC45350

Clone#

2A10

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human FYN expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Mol Cell Biol. 2009 Dec;29(24):6438-48.
2. Cancer Res. 2009 Sep 1;69(17):6889-98.

Product Image

Western Blot

Figure 1: Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.

Flow cytometric

Figure 3: Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa