FYN Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Product Overview
Entrez GenelD
2534
Aliases
SLK; SYN; MGC45350
Clone#
2A10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FYN expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Cell Biol. 2009 Dec;29(24):6438-48.
2. Cancer Res. 2009 Sep 1;69(17):6889-98.
2. Cancer Res. 2009 Sep 1;69(17):6889-98.
Product Image
Western Blot
![Figure 1: Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30229rwb.jpg?v=1666699431)
Figure 1: Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)
Immunohistochemical analysis
![Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30229ihc.jpg?v=1666699431)
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.
Flow cytometric
![Figure 3: Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30229fcm.jpg?v=1666699431)
Figure 3: Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).
Immunofluorescence analysis
![Figure 3: Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30229icc.jpg?v=1666699431)
Figure 3: Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
![Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30229ea.jpg?v=1666699432)
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.