FTL Primary Antibody
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Description
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using FTL mAb against human FTL (AA: FULL(1-157)) recombinant protein. (Expected MW is 45.5 kDa)
Western Blot

Figure 2: Western blot analysis using FTL mAb against HEK293 (1) and FTL (AA: FULL(1-157))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using FTL mouse mAb against K562 cell lysate.
Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of A431 cells using FTL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis

Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FTL mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal tissues using FTL mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.