FTL Primary Antibody

Item Information

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Size

Price

Description

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Product Overview

Entrez GenelD

2512

Aliases

NBIA3

Clone#

8E1E7

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human FTL (AA: FULL(1-157)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Free Radic Biol Med. 2012 May 1;52(9):1692-7.
2. Neurobiol Dis. 2010 Jan;37(1):77-85.

Product Image

Western Blot

Figure 1: Western blot analysis using FTL mAb against human FTL (AA: FULL(1-157)) recombinant protein. (Expected MW is 45.5 kDa)

Western Blot

Figure 2: Western blot analysis using FTL mAb against HEK293 (1) and FTL (AA: FULL(1-157))-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using FTL mouse mAb against K562 cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of A431 cells using FTL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis