FOXO1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | FKH1; FKHR; FOXO1A |
| ProductOrder | F |
| Clone# | 3B6 |
| Entrez GenelD | 2308 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human FOXO1 expressed in E. Coli. |
| MW | 69.7kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Mouse |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 471-600 | |
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2: Western blot analysis using FOXO1 mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)
Western Blot
Figure 3: Western blot analysis using FOXO1 mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of Hela cells using FOXO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.