FOXO1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Product Overview
Entrez GenelD
2308
Aliases
FKH1; FKHR; FOXO1A
Clone#
3B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human FOXO1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
Int J Oncol. 2009 Nov;35(5):1045-51
Cancer Res. 2009 Jul 1;69(13):5433-40.
Cancer Res. 2009 Jul 1;69(13):5433-40.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2: Western blot analysis using FOXO1 mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)
Western Blot
Figure 3: Western blot analysis using FOXO1 mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of Hela cells using FOXO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.