FN1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Product Overview
Entrez GenelD
2335
Aliases
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Clone#
2F4G2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FN1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Pancreas. 2009 Oct;38(7):804-10.
J Biol Chem. 2009 Sep 18;284(38):25879-88.
J Biol Chem. 2009 Sep 18;284(38):25879-88.
Product Image
Western Blot
Figure 1: Western blot analysis using FN1 mAb against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa)
Western Blot
Figure 2: Western blot analysis using FN1 mAb against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using FN1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.