FGFR3 Primary Antibody
|Aliases||ACH; CEK2; JTK4; CD333; HSFGFR3EX|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human FGFR3 (AA: 529-694) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)
Figure 3:Western blot analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of A549 cells using FGFR3 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using FGFR3 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using FGFR3 mouse mAb with DAB staining.
|Description||This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Jul 2009]|
|References (references)||Mol Cancer. 2013 Jul 31;12:83. |
Histochem Cell Biol. 2012 Nov;138(5):759-72.