EZH2 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Product Overview
Entrez GenelD
2146
Aliases
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Clone#
6G4F4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EZH2 (AA: 1-194) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Int J Gynecol Cancer. 2013 Jul;23(6):997-1005.
Cell Cycle. 2013 Jul 1;12(13):2113-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using EZH2 mAb against human EZH2 (AA: 1-194) recombinant protein. (Expected MW is 48.5 kDa)
Figure 2:Western blot analysis using EZH2 mAb against human EZH2 (AA: 1-194) recombinant protein. (Expected MW is 48.5 kDa)
Western Blot
Figure 3:Western blot analysis using EZH2 mAb against HEK293 (1) and EZH2 (AA: 1-194)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using EZH2 mAb against HEK293 (1) and EZH2 (AA: 1-194)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using EZH2 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using EZH2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EZH2 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EZH2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EZH2 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EZH2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.