Mouse Monoclonal Antibody to ETFA

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesEMA; GA2; MADD
Clone#7C2A11
Entrez GenelD2108
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.
MW35kDa
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
134-333
Catalog#: 32246
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)
Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)
Western Blot
Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2),Hek293 (3),Hela (4) and MCF-7 (5) cell lysate.
Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2),Hek293 (3),Hela (4) and MCF-7 (5) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ETFA mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ETFA mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embeddedrectal cancer tissues using ETFA mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embeddedrectal cancer tissues using ETFA mouse mAb with DAB staining.
Product Overview
Description

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

References (references)
References (references)1.Toxicology.2014 Feb 28;316:61-70.2.BMC Med Genomics.2020 Jan 29;13(1):12.