Mouse Monoclonal Antibody to ETFA
Item Information
Catalog #
Size
Price
Description
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
2108
Aliases
EMA; GA2; MADD
Clone#
7C2A11
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Toxicology.2014 Feb 28;316:61-70.2.BMC Med Genomics.2020 Jan 29;13(1):12.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/42349bf7a1f8d16d15a221fb465afb74d043e11dc72ce594a08d6cbad5e120e6.jpg?v=1666706064)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d25961bacbe0390e016b69e6557e38128dcb5b38337425d8aad4623a12673628.jpg?v=1666706064)
Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)
Western Blot
![Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9d9701a7e3aaaecfe56fbe5f92a9a3c819d070785086703130293a53435fab92.jpg?v=1666706064)
Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2),Hek293 (3),Hela (4) and MCF-7 (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7cd369e762688ae50def349f559b29db27e2f8a8f00bc3b388d6db2ae98fce2f.jpg?v=1666706064)
Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2),Hek293 (3),Hela (4) and MCF-7 (5) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/57495b6d5e86dfbe691c324975f0c1dc94f55b500e95850626dac15d3ce209f5.jpg?v=1666706064)
Figure 5:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bc7f8805fb3fba2ec9e2c8402ae306765439964d72606104597eef061a9d4de6.jpg?v=1666706064)
Figure 6:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5905c9e29e970f78ddab348bc1328f7f63bfe007be8cdad2f11e10a127dedd82.jpg?v=1666706064)
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/eb2aabf98ababa56b03c1c0f4f4b1254b4d52187b3609596afdb11d4f7739890.jpg?v=1666706064)
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fc0973f8cf24a2bea14ce6a45b45aaf6cf8c70b18df55603398f1930c00d04b4.jpg?v=1666706064)
Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 10:Immunohistochemical analysis of paraffin-embeddedrectal cancer tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3eeb04204965d43af5bb73146acff05a0e2f4c55d0a5fd5cc327a9953bc8aac5.jpg?v=1666706064)
Figure 10:Immunohistochemical analysis of paraffin-embeddedrectal cancer tissues using ETFA mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.