Mouse Monoclonal Antibody to ETFA
Item Information
Catalog #
Size
Price
Description
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
2108
Aliases
EMA; GA2; MADD
Clone#
2F8A5
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Toxicology.2014 Feb 28;316:61-70.2.BMC Med Genomics.2020 Jan 29;13(1):12.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/df04583ca56336836d205f2c0b51c12c8388a310bdb07137a2efefa04c99c04d.jpg?v=1666706060)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fb16504b1649adaba427e10f1e30832b90d89b7e934998672705605e51e1d562.jpg?v=1666706060)
Figure 2:Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)
Western Blot
![Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a235556eca1b3d1f87d8bd10f174978de564135be67e00a5134dacff07e3b4db.jpg?v=1666706060)
Figure 3:Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2), Hek293 (3),Hela (4)and MCF-7 (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/749b4979d0debb6416133ae2989591c48a66a212e0e87470fec2e132d65d9549.jpg?v=1666706060)
Figure 4:Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2), Hek293 (3),Hela (4)and MCF-7 (5) cell lysate.
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using ETFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/58071caeec543f89e8f1cbfcdf663046f2616ff8dfcafd2c07ff8580f22be6d1.jpg?v=1666706060)
Figure 5:Immunofluorescence analysis of Hela cells using ETFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/338eacf938ce74b5030ba42f34a65ba44b265d2950ffe8fdb606b92b084e8338.jpg?v=1666706060)
Figure 6:Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 7:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ce58b9d3be3513ab0be0a1cdc592d0d49f3b9e0e90e20756a61f58eef4c0e057.jpg?v=1666706060)
Figure 7:Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/830a7b6cd14b40ab7603b029c55be899383a635586c73487a1cfcfacac83db9e.jpg?v=1666706060)
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ETFA mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/33f935f50f138d0a109d4d75dc1c198db528ce24ad53beb508c7f9ef117143f7.jpg?v=1666706060)
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ETFA mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.