ERCC1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesUV20; COFS4; RAD10
ProductOrderE
Clone#1A5A2
Entrez GenelD2067
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ERCC1 (AA: 151-297) expressed in E. Coli.
MW32.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
151-297
Catalog#: 30827
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 151-297) recombinant protein. (Expected MW is 42.5 kDa)
Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 151-297) recombinant protein. (Expected MW is 42.5 kDa)
Western Blot
Figure 3:Western blot analysis using ERCC1 mAb against HEK293 (1) and ERCC1 (AA: 151-297)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ERCC1 mAb against HEK293 (1) and ERCC1 (AA: 151-297)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using ERCC1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using ERCC1 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
References (references)
References (references)1.Tumour Biol. 2014 Sep;35(9):8721-31.
2.Anticancer Res. 2014 Jan;34(1):401-6.