ER Primary Antibody

Item Information
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Description

ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.

Product Overview
Entrez GenelD
2099
Aliases
ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123; ESR1
Clone#
5D4B1
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of ER expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. ampbell, R.A. et al. 2001, J. Biol. Chem. 276, 9817-9824.
2. Chen, D. et al. 2000, Mol. Cell 6, 127-137.
Product Image
Western Blot
Figure 1: Western blot analysis using ER mouse mAb truncated ER recombinant protein (1) MCF-7 cell lysates (2).
Figure 1: Western blot analysis using ER mouse mAb truncated ER recombinant protein (1) MCF-7 cell lysates (2).
Western Blot
Figure 2: Western blot analysis using ER mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ER cDNA (2).
Figure 2: Western blot analysis using ER mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ER cDNA (2).
For Research Use Only. Not for use in diagnostic procedures.