EphB4 Primary Antibody

Item Information
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Price
Description

EPH receptor B4 (EphB4), with 987-amino acid protein (about 108kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptor tyrosine kinases and their ligands, the ephrins, regulate numerous biological processes in developing and adult tissues and have been implicated in cancer progression and in pathological forms of angiogenesis. EphB4 acts as a negative regulator of blood vessel branching and vascular network formation, switching the vascularization program from sprouting angiogenesis to circumferential vessel growth. EphB4 and its ligand ephrinB2 express in several kinds of tumor cells and correlate with tumorigenesis. EphB4 is thus a potential candidate as a predictor of disease outcome in several kinds of tumor and as target for novel therapy.

Product Overview
Entrez GenelD
2050
Aliases
HTK; MYK1; TYRO11
Clone#
5B8F7
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of EphB4 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J. Chrencik, A. Brooun, M. Recht. Structure. 2006 Feb;14(2):321-30.
2. Qinghua WU, Zhenhe SUO,Bjon RISBERG. Pathol Oncol Res. 2004;10(1):26-33.
Product Image
Western Blot
Figure 1: Western blot analysis using EphB4 mouse mAb against truncated EphB4 recombinant protein.
Figure 1: Western blot analysis using EphB4 mouse mAb against truncated EphB4 recombinant protein.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.