EphB3 Primary Antibody

Item Information

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Description

EphB3: EPH receptor B3. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Product Overview

Entrez GenelD

2049

Aliases

ETK2; HEK2; TYRO6

Clone#

4A122D1

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of EphB3 (aa39-212) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Oncogene. 1998 Jan 29;16(4):471-80.
2. Pharmacol Ther. 1998 Mar;77(3):151-81.
3. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9779-84.
4. J Biol Chem. 2002 Jun 21;277(25):23037-43.

Product Image

Western Blot

Figure 1: Western blot analysis using EphB3 mouse mAb against truncated EphB3-His recombinant protein.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A), lung adenocarcinoma (B), colon carcinoma (C), breast carcinoma (D), normal sublingual gland (E), normal rectal (F), showing membrane localization with DAB staining using EphB3 mouse mAb.

Immunohistochemical analysis