EPCAM Primary Antibody

Item Information
Catalog # Size/Concentration Price
32011 100ug $341.00
Specification
AliasesESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Clone#4D6E7
Entrez GeneID4072
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human EPCAM (AA: extra(116-265)) expressed in E. Coli.
MW35kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200-1/1000
FCM (Flow Cytometry)1/200-1/400
WB (Western Blot)1/500 - 1/2000
Sequence
AA: 116-265
Catalog
32011
$341.00
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Images

ELISA

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Cell Culture Products
Figure 2: Western blot analysis using EPCAM mAb against human EPCAM (AA: extra(116-265)) recombinant protein. (Expected MW is 38 kDa)

WESTERN BLOT

Cell Culture Products
Figure 3: Western blot analysis using EPCAM mAb against HEK293-6e (1) and EPCAM (AA: extra(116-265))-hIgGFc transfected HEK293-6e (2) cell lysate.

WESTERN BLOT

Cell Culture Products
Figure 4: Western blot analysis using EPCAM mouse mAb against HCT116 (1), HT-29 (2),SW480 (3),Sw-620 (4) , and T47D (5) cell lysate.

FLOW CYTOMETRY

Cell Culture Products
Figure 5: Flow cytometric analysis of Lovo cells using EPCAM mouse mAb (green) and negative control (red).

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EPCAM mouse mAb with DAB staining.

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 7: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EPCAM mouse mAb with DAB staining.
Product Overview
Description

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

References (references)
References (references) 1,Asian Pac J Cancer Prev. 2020 Mar 1;21(3):861-866. 2,J Radiat Res. 2019 Nov 22;60(6):803-811.

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