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EPCAM Primary Antibody
Item Information | ||
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Catalog # | Size/Concentration | Price |
32010 | 100ug | $358.00 |
Specification | |
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Aliases | ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1 |
Clone# | 6C7F7 |
Entrez GeneID | 4072 |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Host | Mouse |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human EPCAM (AA: extra(116-265)) expressed in E. Coli. |
MW | 35kDa |
Application | |
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ELISA | 1/10000 |
IHC_P (Immunohistochemistry) | 1/200-1/1000 |
FCM (Flow Cytometry) | 1/200-1/400 |
WB (Western Blot) | 1/500 - 1/2000 |
Sequence |
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AA: 116-265 |
Images
ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT

Figure 2: Western blot analysis using EPCAM mAb against human EPCAM (AA: extra(116-265)) recombinant protein. (Expected MW is 38 kDa)
WESTERN BLOT

Figure 3: Western blot analysis using EPCAM mAb against HEK293-6e (1) and EPCAM (AA: extra(116-265))-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT

Figure 4: Western blot analysis using EPCAM mouse mAb against HCT116 (1), HT-29 (2),SW480 (3),Sw-620 (4) , and T47D (5) cell lysate.
FLOW CYTOMETRY

Figure 5: Flow cytometric analysis of Lovo cells using EPCAM mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY

Figure 6: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EPCAM mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY

Figure 7: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using EPCAM mouse mAb with DAB staining.
Product Overview
Description | This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008] |
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References (references)
References (references) | 1,Asian Pac J Cancer Prev. 2020 Mar 1;21(3):861-866. 2,J Radiat Res. 2019 Nov 22;60(6):803-811. |
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