EMD Primary Antibody
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Description
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)
Western Blot
Figure 3:Western blot analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).
Flow cytometric
Figure 6:Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using EMD mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.