EIF5 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).

Product Overview

Entrez GenelD

1983

Aliases

EIF-5; EIF-5A

Clone#

6D6H11

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/50 - 1/250

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Biochemistry. 2006 Apr 11;45(14):4550-8.
2.Biochem Biophys Res Commun. 2011 Dec 2;415(4):567-72.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using EIF5 mAb against human EIF5 (AA: 1-300) recombinant protein. (Expected MW is 60.2 kDa)

Western Blot

Figure 3:Western blot analysis using EIF5 mAb against HEK293 (1) and EIF5 (AA: 1-300)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using EIF5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric