EIF4B Primary Antibody
Item Information
Catalog #
Size
Price
Description
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F
Product Overview
Entrez GenelD
1975
Aliases
EIF-4B; PRO1843
Clone#
1F5
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human EIF4B expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Biol Cell. 2009 Jun;20(11):2673-83.
2. EMBO J. 2006 Jun 21;25(12):2781-91.
2. EMBO J. 2006 Jun 21;25(12):2781-91.
Product Image
Western Blot
![Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365rwb.jpg?v=1666699935)
Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Western Blot
![Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365lwb.jpg?v=1666699935)
Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
Flow cytometric
![Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365fcm.jpg?v=1666699935)
Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
Immunofluorescence analysis
![Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365icc.jpg?v=1666699935)
Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
![Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365ihc.jpg?v=1666699935)
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.
Elisa
![Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30365ea.jpg?v=1666699935)
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.