EIF4B Primary Antibody

Item Information

Catalog #

Size

Price

Description

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F

Product Overview

Entrez GenelD

1975

Aliases

EIF-4B; PRO1843

Clone#

1F5

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human EIF4B expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Mol Biol Cell. 2009 Jun;20(11):2673-83.
2. EMBO J. 2006 Jun 21;25(12):2781-91.

Product Image

Western Blot

Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)

Western Blot

Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.

Flow cytometric

Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis