EIF4B Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesEIF-4B; PRO1843
ProductOrderE
Clone#1F5
Entrez GenelD1975
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human EIF4B expressed in E. Coli.
MW80kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
381-585
Catalog#: 30365
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Images
Western Blot
Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Western Blot
Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F

References (references)
References (references)1. Mol Biol Cell. 2009 Jun;20(11):2673-83.
2. EMBO J. 2006 Jun 21;25(12):2781-91.