EIF2AK2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPKR; PRKR; EIF2AK1
ProductOrderE
Clone#6H3A10
Entrez GenelD5610
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli.
MW62kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
329-551
Catalog#: 30680
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Images
Western Blot
Figure 1: Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Figure 1: Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot
Figure 2: Western blot analysis using EIF2AK2 mAb against HEK293 (1) and EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using EIF2AK2 mAb against HEK293 (1) and EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.
Figure 3: Western blot analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

References (references)
References (references)1. J Alzheimers Dis. 2010;21(4):1217-31.
2. Mol Cells. 2011 Aug;32(2):167-72.