EIF2A Primary Antibody
Item Information
Catalog #
Size
Price
Description
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes
Product Overview
Entrez GenelD
83939
Aliases
CDA02; EIF-2A; MST089; MSTP004; MSTP089
Clone#
3A7A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EIF2A (AA: 448-576) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Biol (Mosk). 2010 Sep-Oct;44(5):859-66.
2. Cancer Res. 2009 Feb 15;69(4):1545-52.
2. Cancer Res. 2009 Feb 15;69(4):1545-52.
Product Image
Western Blot
Figure 1: Western blot analysis using EIF2A mAb against human EIF2A (AA: 448-576) recombinant protein. (Expected MW is 40.3 kDa)
Western Blot
Figure 2: Western blot analysis using EIF2A mAb against HEK293 (1) and EIF2A (AA: 448-576)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2A mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.