E2F1 Primary Antibody

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Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Figure 1: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
Figure 2: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Figure 2: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 30384

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