E2F1 Primary Antibody
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
2.Mol Cancer Ther. 2010 May;9(5):1265-73.
![Figure 1: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384rwb.jpg?v=1666699987)
![Figure 2: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384lwb.jpg?v=1666699987)
![Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384ihc-1.jpg?v=1666699987)
![Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384ihc-2.jpg?v=1666699987)
![Figure 5: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384fcm.jpg?v=1666699987)
![Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30384ea.jpg?v=1666699987)