Dynamin-2 Primary Antibody
Item Information
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Size
Price
Description
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
Product Overview
Entrez GenelD
1785
Aliases
DNM2;DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
Clone#
3F5F3
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human Dynamin-2 (AA: 520-744) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/250 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Med. 2014 Feb;3(1):14-24.
2.Eur J Obstet Gynecol Reprod Biol. 2012 Oct;164(2):180-4.
2.Eur J Obstet Gynecol Reprod Biol. 2012 Oct;164(2):180-4.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using Dynamin-2 mAb against human Dynamin-2 (AA: 520-744) recombinant protein. (Expected MW is 28 kDa)
Western Blot
Figure 3:Western blot analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using Dynamin-2 mouse mAb against Hela (1), U251 (2), K562 (3), Jurkat (4), and Ramos (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using *** mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.