DNMT3B Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesICF; ICF1; M.HsaIIIB
ProductOrderD
Clone#7E5E9C1
Entrez GenelD1789
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.
MW95.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-150
Catalog#: 30855
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using DNMT3B mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using DNMT3B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DNMT3B mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DNMT3B mouse mAb with DAB staining.
Product Overview
DescriptionCpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
References (references)
References (references)1.PLoS One. 2013 Jul 19;8(7):e69486.
2.Blood Coagul Fibrinolysis. 2012 Oct;23(7):636-9.