DNMT3B Primary Antibody

Item Information
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Size
Price
Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Product Overview
Entrez GenelD
1789
Aliases
ICF; ICF1; M.HsaIIIB
Clone#
4D1A2E5
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2013 Jul 19;8(7):e69486.
2.Blood Coagul Fibrinolysis. 2012 Oct;23(7):636-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 4:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 7:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.