DNMT3B Primary Antibody
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 5:Immunofluorescence analysis of HeLa cells using DNMT3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.