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DFFB Primary Antibody
|Aliases||CAD; CPAN; DFF2; DFF40; DFF-40|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|IHC_P (Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
|(AA: 1-289) expressed in E. Coli.|
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2: Western blot analysis using DFFB mAb against human DFFB (AA: 1-289) recombinant protein. (Expected MW is 59.4 kDa)
Figure 3: Western blot analysis using DFFB mAb against HEK293 (1) and DFFB (AA: 1-289)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4: Flow cytometric analysis of Hela cells using DFFB mouse mAb (green) and negative control (red).
|Tissue Array Results|
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Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.
|References (references)||1.PLoS One. 2012;7(10):e45686.
2.J Mol Biol. 2011 Feb 25;406(3):355-61.