DFFB Primary Antibody

Item Information
Catalog # Size/Concentration Price
31736 100ug $341.00
Specification
AliasesCAD; CPAN; DFF2; DFF40; DFF-40
Clone#2E6G3
Entrez GeneID1677
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenMouse IgG1
MW218.4kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) Purified recombinant fragment of human MCM3AP (AA: 293-442) expressed in E. Coli.
FCM (Flow Cytometry)1/200 - 1/400
Sequence
(AA: 1-289) expressed in E. Coli.
Catalog
31736
$341.00
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Images

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Cell Culture Products

Figure 2: Western blot analysis using DFFB mAb against human DFFB (AA: 1-289) recombinant protein. (Expected MW is 59.4 kDa)

Cell Culture Products

Figure 3: Western blot analysis using DFFB mAb against HEK293 (1) and DFFB (AA: 1-289)-hIgGFc transfected HEK293 (2) cell lysate.

Cell Culture Products

Figure 4: Flow cytometric analysis of Hela cells using DFFB mouse mAb (green) and negative control (red).

Cell Culture Products
Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
E9/10 Endometrial Cancer
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Product Overview
Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

References (references)
References (references) 1.PLoS One. 2012;7(10):e45686.
2.J Mol Biol. 2011 Feb 25;406(3):355-61.

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