DDX4 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesVASA;MGC111074;DDX4
ProductOrderD
Clone#2F9H5
Entrez GenelD54514
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human DDX4 expressed in E. Coli.
MW76kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-160
Catalog#: 20408
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Images
Western Blot
Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
Product Overview
Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

References (references)
References (references)1. Proc Natl Acad Sci USA.2000 97(17):9585-90
2. Lab Invest.2002 82(2):159-66
3. Mol Reprod Dev.2004 67(1):1-7
4. Nat Genet.2004 36(1):40-5