DDX4 Primary Antibody
Item Information
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Size
Price
Description
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Product Overview
Entrez GenelD
54514
Aliases
VASA;MGC111074;DDX4
Clone#
2F9H5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Proc Natl Acad Sci USA.2000 97(17):9585-90
2. Lab Invest.2002 82(2):159-66
3. Mol Reprod Dev.2004 67(1):1-7
4. Nat Genet.2004 36(1):40-5
2. Lab Invest.2002 82(2):159-66
3. Mol Reprod Dev.2004 67(1):1-7
4. Nat Genet.2004 36(1):40-5
Product Image
Western Blot
Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.