DDX4 Primary Antibody

Item Information

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Size

Price

Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Product Overview

Entrez GenelD

54514

Aliases

VASA;MGC111074;DDX4

Clone#

2F9H5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Proc Natl Acad Sci USA.2000 97(17):9585-90
2. Lab Invest.2002 82(2):159-66
3. Mol Reprod Dev.2004 67(1):1-7
4. Nat Genet.2004 36(1):40-5

Product Image

Western Blot

Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Flow cytometric