DCX Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDC; DBCN; LISX; SCLH; XLIS
ProductOrderD
Clone#2G5
Entrez GenelD1641
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human DCX (AA: 362-411) expressed in E. Coli.

MW49.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
362-411
Catalog#: 30381
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Images
Western Blot
Figure 1: Western blot analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)
Figure 1: Western blot analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)
Western Blot
Figure 2: Western blot analysis using DCX mouse mAb against Mouse heart (1) lysate.
Figure 2: Western blot analysis using DCX mouse mAb against Mouse heart (1) lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissue tissues using DCX mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissue tissues using DCX mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using DCX mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using DCX mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 5: Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 6: Flow cytometric analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).
Figure 6: Flow cytometric analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.FASEB J. 2009 Dec;23(12):4276-87.
2.J Cell Biol. 2010 Nov 1;191(3):463-70.